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inverted epifluorescence microscope te2000- e- pfs  (Nikon)


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    Structured Review

    Nikon inverted epifluorescence microscope te2000- e- pfs
    Inverted Epifluorescence Microscope Te2000 E Pfs, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inverted+microscope+te2000+pfs/pm39869799-450-10-18
    Average 90 stars, based on 1 article reviews
    inverted epifluorescence microscope te2000- e- pfs - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Inverted Microscopy:

    Article Title: Cytoskeletal forces during signaling activation in Jurkat T-cells
    Article Snippet: .. Images were collected using an inverted microscope (TE2000 PFS; Nikon, Melville, NY) with a cooled charge-coupled device camera (CoolSNAP HQ2; Photometrics, Tucson, AZ). .. EGFP-actin and fluorescent receptors were imaged with total internal reflection fluorescence using a 60×/1.49 numerical aperture objective lens, a 491-nm laser (100 mW; Andor, South Windsor, CT) for EGFP excitation, and a 561-nm laser (75 mW; Andor) for FluoSphereRed excitation.

    Article Title: Adhesion-dependent modulation of actin dynamics in Jurkat T cells.
    Article Snippet: Contact formation of T cells with antigen presenting cells results in the engagement of T cell receptors (TCRs), recruitment and aggregation of signaling proteins into microclusters and ultimately, T cell activation.. During this process, T cells undergo dramatic changes in cell shape and reorganization of the cytoskeleton.. While the importance of the cytoskeleton in T cell activation is well known, the dynamics of the actin cytoskeleton and how it correlates with signaling clusters during the early stages of spreading is not well understood.

    Article Title: Ligand Mobility Regulates B Cell Receptor Clustering and Signaling Activation
    Article Snippet: .. Images were collected using an inverted microscope (TE2000 PFS, Nikon, Melville, NY) with a cooled CCD camera (Coolsnap HQ2, Photometrics, Tucson, AZ) at a frame interval of 3 s. EGFP-actin and fluorescent receptors were imaged with total internal reflection fluorescence (TIRF) using a 60 × 1.49 NA objective lens, a 491 nm laser (100 mW, Andor, South Windsor, CT) for EGFP excitation, and a 561 nm laser (75 mW, Andor) for AF546 excitation. ..

    Article Title: Topography on a subcellular scale modulates cellular adhesions and actin stress fiber dynamics in tumor associated fibroblasts.
    Article Snippet: Cells can sense and adapt to mechanical properties of their environment.. The local geometry of the extracellular matrix, such as its topography, has been shown to modulate cell morphology, migration, and proliferation.. Here we investigate the effect of micro/nanotopography on the morphology and cytoskeletal dynamics of human pancreatic tumor-associated fibroblast cells (TAFs).

    Article Title: The actin crosslinking protein palladin modulates force generation and mechanosensitivity of tumor associated fibroblasts
    Article Snippet: .. Fluorescence and Interference Reflection Microscopy images were collected at 37 °C using an inverted microscope (TE2000 PFS, Nikon, Melville, NY) with a cooled CCD camera (Coolsnap HQ2, Photometrics, Tucson, AZ). .. TIRF imaging was done using a 60x magnification, 1.49 NA objective lens, a 491 nm laser (100 mW, Andor, South Windsor, CT) for EGFP excitation and a 561 nm laser (75 mW, Andor) for mApple and mCherry excitation.

    Article Title: Subcellular topography modulates actin dynamics and signaling in B-cells
    Article Snippet: .. Images were collected using an inverted microscope (TE2000 PFS; Nikon, Melville, NY) with a cooled charge-coupled device camera (Coolsnap HQ2; Photometrics, Tucson, AZ) at a frame interval of 3 s. EGFP-actin and fluorescent receptors were imaged with TIRF using the 60×, 1.49 NA objective lens, the 491-nm laser for EGFP excitation, and a 561-nm laser (75 mW; Cobolt Jive) for AF546 excitation. ..

    Fluorescence:

    Article Title: Adhesion-dependent modulation of actin dynamics in Jurkat T cells.
    Article Snippet: Contact formation of T cells with antigen presenting cells results in the engagement of T cell receptors (TCRs), recruitment and aggregation of signaling proteins into microclusters and ultimately, T cell activation.. During this process, T cells undergo dramatic changes in cell shape and reorganization of the cytoskeleton.. While the importance of the cytoskeleton in T cell activation is well known, the dynamics of the actin cytoskeleton and how it correlates with signaling clusters during the early stages of spreading is not well understood.

    Article Title: Ligand Mobility Regulates B Cell Receptor Clustering and Signaling Activation
    Article Snippet: .. Images were collected using an inverted microscope (TE2000 PFS, Nikon, Melville, NY) with a cooled CCD camera (Coolsnap HQ2, Photometrics, Tucson, AZ) at a frame interval of 3 s. EGFP-actin and fluorescent receptors were imaged with total internal reflection fluorescence (TIRF) using a 60 × 1.49 NA objective lens, a 491 nm laser (100 mW, Andor, South Windsor, CT) for EGFP excitation, and a 561 nm laser (75 mW, Andor) for AF546 excitation. ..

    Article Title: Topography on a subcellular scale modulates cellular adhesions and actin stress fiber dynamics in tumor associated fibroblasts.
    Article Snippet: Cells can sense and adapt to mechanical properties of their environment.. The local geometry of the extracellular matrix, such as its topography, has been shown to modulate cell morphology, migration, and proliferation.. Here we investigate the effect of micro/nanotopography on the morphology and cytoskeletal dynamics of human pancreatic tumor-associated fibroblast cells (TAFs).

    Article Title: The actin crosslinking protein palladin modulates force generation and mechanosensitivity of tumor associated fibroblasts
    Article Snippet: .. Fluorescence and Interference Reflection Microscopy images were collected at 37 °C using an inverted microscope (TE2000 PFS, Nikon, Melville, NY) with a cooled CCD camera (Coolsnap HQ2, Photometrics, Tucson, AZ). .. TIRF imaging was done using a 60x magnification, 1.49 NA objective lens, a 491 nm laser (100 mW, Andor, South Windsor, CT) for EGFP excitation and a 561 nm laser (75 mW, Andor) for mApple and mCherry excitation.

    Microscopy:

    Article Title: Topography on a subcellular scale modulates cellular adhesions and actin stress fiber dynamics in tumor associated fibroblasts.
    Article Snippet: Cells can sense and adapt to mechanical properties of their environment.. The local geometry of the extracellular matrix, such as its topography, has been shown to modulate cell morphology, migration, and proliferation.. Here we investigate the effect of micro/nanotopography on the morphology and cytoskeletal dynamics of human pancreatic tumor-associated fibroblast cells (TAFs).

    Article Title: The actin crosslinking protein palladin modulates force generation and mechanosensitivity of tumor associated fibroblasts
    Article Snippet: .. Fluorescence and Interference Reflection Microscopy images were collected at 37 °C using an inverted microscope (TE2000 PFS, Nikon, Melville, NY) with a cooled CCD camera (Coolsnap HQ2, Photometrics, Tucson, AZ). .. TIRF imaging was done using a 60x magnification, 1.49 NA objective lens, a 491 nm laser (100 mW, Andor, South Windsor, CT) for EGFP excitation and a 561 nm laser (75 mW, Andor) for mApple and mCherry excitation.



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